Table 5.

Primers.

Primer ID	Sequence	Details
PSPGOXF	5' - TTTTTGCTGTCATTCGTTCAAGGTAAAAGAAGCAATGGCATTGAAGCC	Underlined sequence is homologous to the last bases of K. lactis killer protein k1 secretion signal sequence
PSPGOXR	5' - CACCACCACCAGTAGAGACATGGGAGATCGTCACTGCATGGAAGCATA	Underlined sequence is homologous to the first bases of S. cerevisiae PGK terminator
PINU1Fb	5'- GGATCCGAATTCTCAAACCGAAATGGG	Underlined extremity is an added BamHI restriction site
PINU1Rb	5'- GGATCCACGCCAGACGTCTTGTGTCCG	Underlined extremity is an added BamHI restriction site
PINUGOXF	5' -GTCAGTGCTTCAGTTATCAATTACAAGAGA AGCAATGGCATTGAAGCC	Underlined sequence is homologous to the last bases of K. marxianus INU1 secretion signal sequence
PINUGOXR	5'- TTTTGTCGTTAGTAAAGTAAGCAGATCAGA TCACTGCATGGAAGCATA	Underlined sequence is homologous to the last bases of K. marxianus INU1 terminator

List of primers used on DNA constructions.