Figure 3.

ASPM knockdown in glioblastoma gliomaspheres. Cell kinetics of GBM1 (a) and GBM2 (b) gliomaspheres. The data were generated from three independent experiments. Gliomaspheres treated with sh-miR-Aspm do not grow as compared to non-transduced gliomaspheres and gliomaspheres transduced with non-silencing sh-miR-RNA, which start growing again after a transient drop; (c, d) at day 33, there is extensive cell death in cell populations of GBM1 and GBM2 expressing the ASPM-silencing sh-miR-RNA as compared to the cells expressing a non-targeted sh-miR-RNA.