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Cell death and proliferation inhibition induced by ASPM silencing, evaluated by 7-Actinomycin D (7-AAD) labeling and 5- Ethyl -2'- Deoxyuridine (EdU) incorporation. Compared to control (a), transduction of GBM1 with sh-miR-Aspm results in massive cell death (b) and a marked decrease in proliferation of surviving cells, with only 25% of the viable cells being EdU+ (d), compared to 64% for the controls (c).
