Skip to main content
. 2010 Feb 9;5(2):e9069. doi: 10.1371/journal.pone.0009069

Figure 7. Effect of berberine on association of endogenous HuR with TNF-α and IL-6 in HIV PI-treated macrophages.

Figure 7

Mouse J774A.1 cells were pretreated with berberine (BBR, 0.5 µg/ml) for 2 h and then treated with lopinavir (LOPV, 15 µM) for 24 h. Total cell lysates were subjected to immunoprecipitation (IP) using either a HuR Ab or an isotype-matched control IgG1. mRNA levels in IP materials were detected by real-time RT-PCR using gene-specific primers as described in “Methods” and expressed as fold enrichment of mRNA in HuR IP over control IgG1 IP. Values are mean ± SD of three independent experiments. Statistical significance relative to vehicle control, *p<0.05. Statistical significance of the same HIV PI treatment between control group and BBR group, # p<0.05.