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. 2010 Feb 9;5(2):e9026. doi: 10.1371/journal.pone.0009026

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Jee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Functional categorization of genes in de-ATSC. (B) Commonly expressed genes between the de-ATSC and hESC. Also, many kinds of histones and DNA methylation-related transcription factors and enzymes are overexpressed after ATSC de-differentiation (Table). (C) Differential embryonic genes, Utf1, Dapp5, FGF4, and Eras expressions in de-ATSCs. (D) Evaluation of epigenetic modifications through methylation analysis on promoter regions through bisulfite modification and sequencing of genomic DNA. The related method was explained in Materials and Methods S1.