Figure 5. Determination of adipogenic, osteogenic, and muscle differentiation potencies of de-ATSC.
In vitro (A) and in vivo Determination of adipogenic, osteogenic, and muscle differentiation potencies of de-ATSC SCID/NOD mice (B). The transplants were recovered 6 weeks later, stained via Alzarin Red (bone), Masson (muscle, chondrocyte), and Van Gieson (chondrocyte) and collagen IV immunostaining. (C) Teratoma formation from engrafted de-ATSCs in SCID mouse and development of three germ layer-derived tissue or organ such as muscle, neuron, pigment cell, and gland in teratoma after subcutaneously implantation in 8-week-old immunodeficient beige mice. The paraffin-embedded sections were stained via H&E, Alzarin Red (bone), Masson (muscle and chondrocytes), and van Gieson (chondrocytes). Datas presented are presented as mean ±SD; n>4. * p % 0.05, and ** p % 0.01, Student's t test.