Table 1. Basic characteristics of the selected microsatellite markers.
Panel | Marker | Chr: position | Repeat unit | Labeled primer sequence (5′-3′) | Unlabeled primer sequence (5′-3′)* | Conc. (μM) | No. alleles (range) |
CNA2 | CNA2A | 11: 389201–389350 | CT | FAM- CGAGGTCATGTTGTGAGTCC | G TGACCGTCTCGTTCTTCTCA | 0.3 | 15 (10–60) |
CNA2B | 9: 191563–191711 | TG | HEX- TCGTCAACGATGCAAGTCTC | GGGCCTGGGAAATAGGTAGA | 0.3 | 6 (8–21) | |
CNA2C | 10: 307773–306912 | TA | TET- AGAAGCACATGGGGAAAGG | GCGCAGTTTGAAGATGAGAA | 1.0 | 16 (8–44) | |
CNA3 | CNA3A | 11: 1281429–12814716 | CTA | FAM- ACCCCCTGCCCATCATA | GCACAGGCATAAAGCTAAGTGTGA | 0.3 | 9 (19–69) |
CNA3B | 4: 339525–339664 | TCT | HEX- TGGGGATATCGATTCCTTCTC | GATTGGTATGGGAAGCGTTG | 0.3 | 5 (5–17) | |
CNA3C | 7: 285123–285270 | CCA | TET- TGGAAGAGGATGGAGCGTAT | GCATAGTTTATCGTTTTCTCTTTTC | 0.3 | 10 (8–38) | |
CNA4 | CNA4A | 5: 233120–233445 | TTAT | FAM- CGTCGAAGACTGCACAAAAA | GTTCTGTATGACAGGTCGCAAA | 1.0 | 51 (15–119) |
CNA4B | 4: 1021855–1022020 | ATCC | HEX- CGGATGAGATGGAAAGAAGG | GTGCGTCTGTCAAAAGATTGC | 0.3 | 10 (5–25) | |
CNA4C | 14: 131866–132031 | TATT | TET- AGATGTCCTGGCGATGTTG | GAGGAGCAAGCAATCAAACC | 0.3 | 11 (1–18) |
The underlined residue(s) are not a match to the genomic sequence. These were introduced to minimize the formation of minus A peaks, a well known PCR artifact that may complicate interpretation of the results [34].