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. 2010 Feb 9;5(2):e9124. doi: 10.1371/journal.pone.0009124

Table 1. Basic characteristics of the selected microsatellite markers.

Panel Marker Chr: position Repeat unit Labeled primer sequence (5′-3′) Unlabeled primer sequence (5′-3′)* Conc. (μM) No. alleles (range)
CNA2 CNA2A 11: 389201–389350 CT FAM- CGAGGTCATGTTGTGAGTCC G TGACCGTCTCGTTCTTCTCA 0.3 15 (10–60)
CNA2B 9: 191563–191711 TG HEX- TCGTCAACGATGCAAGTCTC GGGCCTGGGAAATAGGTAGA 0.3 6 (8–21)
CNA2C 10: 307773–306912 TA TET- AGAAGCACATGGGGAAAGG GCGCAGTTTGAAGATGAGAA 1.0 16 (8–44)
CNA3 CNA3A 11: 1281429–12814716 CTA FAM- ACCCCCTGCCCATCATA GCACAGGCATAAAGCTAAGTGTGA 0.3 9 (19–69)
CNA3B 4: 339525–339664 TCT HEX- TGGGGATATCGATTCCTTCTC GATTGGTATGGGAAGCGTTG 0.3 5 (5–17)
CNA3C 7: 285123–285270 CCA TET- TGGAAGAGGATGGAGCGTAT GCATAGTTTATCGTTTTCTCTTTTC 0.3 10 (8–38)
CNA4 CNA4A 5: 233120–233445 TTAT FAM- CGTCGAAGACTGCACAAAAA GTTCTGTATGACAGGTCGCAAA 1.0 51 (15–119)
CNA4B 4: 1021855–1022020 ATCC HEX- CGGATGAGATGGAAAGAAGG GTGCGTCTGTCAAAAGATTGC 0.3 10 (5–25)
CNA4C 14: 131866–132031 TATT TET- AGATGTCCTGGCGATGTTG GAGGAGCAAGCAATCAAACC 0.3 11 (1–18)
*

The underlined residue(s) are not a match to the genomic sequence. These were introduced to minimize the formation of minus A peaks, a well known PCR artifact that may complicate interpretation of the results [34].