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. 2010 Feb 9;5(2):e9132. doi: 10.1371/journal.pone.0009132

Figure 1. GRN163 inhibits telomerase activity in a sequence specific manner and shortens telomeres.

Figure 1

a. SK-N-MC, MCF-7 and K562 were exposed to 5uM of GRN163. Telomerase activity was assessed by the TRAP assay after 24hours. C- control untreated cells, M- mismatch nonspecific scrambled oligo, I-telomerase inhibitor GRN163. R8- standard TRAP control, N- negative control with no cell extracts, IC- internal PCR control. The extent of telomerase inhibition is denoted in percentages below the lanes. b. SK-N-MC cells were continuously exposed to 5µM of GRN163. Telomere length was measured by Southern blot. C- control untreated cells, Ia- telomere length of cells exposed to telomerase inhibitor for three months, Ib- telomere length of cells exposed to telomerase inhibitor for 16 months, M- molecular size marker. Mean telomere length is denoted below each lane, and the percent of telomere shortening is shown as well. c. Graphical presentation of the extent of telomere shortening after the exposure of SK-N-MC cells to GRN163 for 1 year as measured by Southern blot.