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. 2010 Feb 9;5(2):e9135. doi: 10.1371/journal.pone.0009135

Figure 1. γ-secretase dependence of the APPGV16/Gal4-luciferaseEGFP/EF1- NLS-βGal reporter cells.

Figure 1

The N2a EF1-APPGV16/Gal4-luciferaseEGFP/EF1-NLS-βGal stable cells (NAG cells) have γ-secretase dependent EGFP expression, as DAPT eliminates the majority of EGFP fluorescence (A). The Gal4-luciferase moiety of the reporter is also γ-secretase dependent as titrated DAPT diminishes luciferase activity (B). The decrease in luciferase activity correlates with elevated CTFGV16 protein levels across the DAPT titration (C).