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Plasmids used for transformation of A. marginale St. Maries. (Panel A) Plasmid pET28 Am tr Himar transposase encoding the A7 allele of himar1, driven by the A. marginale transcriptional regulator promoter, tr. (Panel B) Plasmid pHimar Turbo-SS encoding the TurboGFP gene and the spectinomycin–streptomycin resistance gene, both driven by the tr promoter. These constitute the transposon that is flanked by the left and right Himar repeats which are recognized by the transposase.
