Fig. 2.

Insertion site map of the Anaplasma marginale transformant, AmStMTSS. The two X-marks denote the boundaries of the plasmid integrated in its entirety into the A. marginale chromosome at nucleotide position 1026363. Angled arrows indicate the start sites for binding of the primers shown. Primer pair AmTSS PCR A FOR and AmTSS PCR A REV direct the Taq polymerase to read across the upstream junction, whereas primer pair AmTSS PCR B FOR and AmTSS PCR B REV direct reading across the downstream junction between the plasmid and wild type A. marginale DNA. PCR using primers AmTSS PCR A FOR and AmTSS PCR B REV direct the polymerase to read across the entire plasmid insert and flanking regions.