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. 2010 Feb 10;167(2-4):167–174. doi: 10.1016/j.vetpar.2009.09.018

Fig. 5.

Fig. 5

Gel image of PCR results comparing wild type (WT AmStM lanes) and transformed A. marginale (AmStMTSS lanes) DNA extracts. (Lane 1) 2-kb ladder; (lanes 2 and 3) DNA amplified using primers AmTSS PCR A FOR and AmTSS PCR A REV (A FOR–A REV). The result confirms integration of the plasmid (pHimar Turbo-SS) downstream from the A. marginale ndk gene, coding for nucleoside diphosphate kinase; (lanes 4 and 5) DNA amplified using primers AmTSS PCR B FOR and AmTSS PCR B REV (B FOR–B REV). The result confirms presence of pHimar Turbo-SS sequences downstream from the E. coli origin of replication (pUC ori) adjacent to the wild type A. marginale tr promoter and the Am tr DNA binding protein gene; (lanes 6 and 7) DNA amplified using primers Turbo 5′ CON PCR and Turbo 3′ CON PCR (TurboGFP) to confirm presence of the TurboGFP gene; (lanes 8 and 9) DNA amplified using primers T-SS CON PCR FOR and T-SS CON PCR REV (T-SS). The result confirms presence of both TurboGFP and resistance gene sequences in the correct position to each other; (lane 10) 100-bp DNA ladder.