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Hypercholesterolemic serum could alter the distribution of ZO-1 in endothelial cells, which seemed to be dose dependent. PI3K specific inhibitor wortmannin could significantly reverse the effect induced by hypercholesterolemic serum. (a) serum free medium; (b) normal rabbit serum; (c) low concentration of hypercholesterolemic serum (0.04 mMol/L); (d) high concentration of hypercholesterolemic serum (0.32 mMol/L); (e) c plus 100 nMol/L wortmannin; (f) d plus 100 nMol/L wortmannin.
