Figure 4.

Protection of PpiD* from amide NH exchange. (A) The protection factors derived from H/D exchange experiments in 100 mM K phosphate (pH 7.0) at 25°C are plotted as a function of the amino acid sequence. The secondary structure elements are indicated at the top of the figure. (B) Protection factors higher than 1000 are mapped in blue on the backbone structure, protection factors between 100 and 1000 in cyan, and residues exchanging in the dead time of the experiment are colored in red. Residues lacking an amide proton are colored in gray.