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19F NMR spectrum of N-terminal domain (residues 1-191) of (A) wt-apoE4 and (B) wt-apoE3. Data collected at 22°C in 20 mM HEPES, 80 mM NaCl, 0.1% βME at pH 7.4. The protein concentrations were 30 μM. The peaks at −48.4 ppm in A and −48.9 ppm in B are assigned to Trp39 [see Supporting Information Fig. 1(A)].
