Figure 5.

Urea denaturation of the apoE4-C-terminal mutant. Experiments performed in 20 mM HEPES, 80 mM NaCl, 0.1% βME at pH 7.4 and 22 °C. A 10 M stock urea solution in 20 mM Hepes buffer, pH 7.4 was added to the solution in appropriate amounts to make 1, 2, 3, 4, and 5 M urea solutions. The protein concentration at 0 M urea was 60 μM. The data shown are corrected for dilution.