Table II.
Kinetic Parameters of wtTAP and Its H135 TAP Variants Using pNPP as Substrate With 10 mM MgCl2 and 1 mM ZnCl2 at 25°C
| Enzymes | Km (mM) | kcat (s−1) | kcat/Km (s−1·mM−1) |
|---|---|---|---|
| WT | 0.312 | 50.04 | 160.4 |
| H135D | 3.091 | 101.5 | 32.8 |
| H135E | 2.874 | 146 | 48.7 |
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