Table V.
Thermal inactivation of wtTAP and the H135 TAP variants at 55°C and 60°C
| Activity (%) |
|||
|---|---|---|---|
| wtTAP | H135D-TAP | H135E-TAP | Heat treatment/min |
| At 55°C | |||
| 0 | 100 | 100 | 100 |
| 5 | 17.5 | 79.8 | 4 |
| 10 | 14.2 | 76 | 2.4 |
| 20 | 11.2 | 65.6 | 1.4 |
| 60 | 6.6 | 56.1 | 0.8 |
| 120 | 3.7 | 42 | 0.3 |
| At 60°C | |||
| 0 | 100 | 100 | 100 |
| 5 | 9.9 | 30.5 | 0.7 |
| 10 | 6.3 | 17 | 0.5 |
| 20 | 3.2 | 9.7 | 0.4 |
| 60 | 1.3 | 4.4 | 0.2 |
| 120 | 0.6 | 2.3 | 0.09 |
Reported values are the average of at least three measurements, and standard deviations do not exceed 5%.