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Immunoblot analysis of β2-AR expressed in COS-1 cells using the monoclonal antibody rho-1D4. Immunoblot of membranes expressing the wild-type (WT) β2-AR and Val114 mutants (around 5 μg of solubilized membrane protein was loaded), the single lane shows PNGaseF treated WT β2-AR. The arrow indicates the fully glycosylated receptor. Mobility of molecular weight standards in kilo Daltons is indicated next to the gel.
