Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jan 19;8(1):80–90. doi: 10.3390/md8010080

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Suppression of Tfn endocytosis by geoditin A. HT29 cells were treated with geoditin A for 24 h, washed with warm plain DMEM medium with 1% BSA, incubated with fresh DMEM containing 50 μg/mL Alexa-594 Tfn for 60 min, before the cellular uptake of Tfn was observed under a fluorescence microscope. Representative images were captured using FV1600 Olympus system. Tfn was located in endosomes of untreated cells (a) but decreased in a dose-dependent manner after treatment with geoditin A for 24 h (b, c, d for 10, 20 and 40 μg/mL, respectively). About 30% decrease of Alexa-594 Tfn was measured in HT29 cells treated with geoditin A for 40 μg/mL by flow cytometry (e).

Suppression of Tfn endocytosis by geoditin A. HT29 cells were treated with geoditin A for 24 h, washed with warm plain DMEM medium with 1% BSA, incubated with fresh DMEM containing 50 μg/mL Alexa-594 Tfn for 60 min, before the cellular uptake of Tfn was observed under a fluorescence microscope. Representative images were captured using FV1600 Olympus system. Tfn was located in endosomes of untreated cells (a) but decreased in a dose-dependent manner after treatment with geoditin A for 24 h (b, c, d for 10, 20 and 40 μg/mL, respectively). About 30% decrease of Alexa-594 Tfn was measured in HT29 cells treated with geoditin A for 40 μg/mL by flow cytometry (e).