Table 2.

Recombinant agarases from engineered microorganisms: localization, characteristics, products, and accession number.

Source	Expression strain	Name of the gene	Localization	Production		GH Family	Mr (kDa)	Specific activity (U/mg)	Optimal T (°C)	Stable up to T (°C)	Optimal pH	Stable pH	Product	GeneBank accession number	Ref.
				(U/L)	(mg/L)
β-agarase
Pseudomonas sp. W7	Escherichia coli JM83	-	extracellular	-		16	59	-	20–40	-	7.8	-	NA4	AAF82611	[39]
Vibrio sp. PO-303	Escherichia coli BL21	agaA	intracellular	-		16	106	16.4	40	-	7.5	-	NA4, NA6	BAF62129	[7]
Vibrio sp. PO-303	Escherichia coli DH5α	agaD	intracellular	620	10	16	51	63.6	40	45	7.5	4–9	NA4(main), NA2, NA6	BAF34350	[40]
Vibrio sp. PO-303	Escherichia coli BL21	agaC	intracellular	7130	22	86	51	329	35	37	6.0	4–8	NA4, NA6, NA8(main)	BAF03590	[47]
Agarivorans sp. JA-1	Escherichia coli DH5α	-	intracellular	554	3	50	109	167	40	60 (70%)	8.0	-	NA2, NA4	ABK97391	[42]
Saccharophagus degradans 2–40	Escherichia coli EPI300	aga50A	intracellular	-		50	87	-	-	-	-	-	-	ABD80438	[34]
		aga16B				16	64							ABD80437
		aga86C				86	86							ABD81910
		Aga50D				50	89							ABD81904
		aga86E				86	146							ABD81915
Microbulbifer-like JAMB-A94	Bacillus subtilis	agaO	extracellular	7816	80	86	127	98	45	40	7.5	6–9	NA6 (main)	BAD86832	[35]
Zobellia galactanivorans	Escherichia coli DH5α	agaA	intracellular	160	1	16	60	160	-	-	-	-	NA4, NA6	AAF21820	[43]
		agaB		800	8		31	100					NA2, NA4	AAF21821
Agarivorans sp. JAMB-A11	Bacillus subtilis	agaA11	extracellular	19000	51	50	105	371	40	40	7.5–8.0	6–11	NA2 (main)	BAD99519	[45]
Microbulbifer thermotolerans JAMB-A94	Bacillus subtilis	agaA	extracellular	45000	87	16	48	517	55	60	7.0	6–9	NA4 (main)	BAD29947	[46]
Microbulbifer sp. JAMB-A7	Bacillus subtilis	agaA7	extracellular	25831	65	16	49	398	50	50	7.0	5–8	NA4	BAC99022	[36]
Pseudomonas sp. SK38	Escherichia coli BL21	pagA	intracellular	-		16	37	32.3	30	37	9.0	8–9	-	AF534639	[48]
Vibrio sp. JT0107	Escherichia coli DH5α	agaA	intracellular	-		50	105	-	-	-	-	-	-	BAA03541	[50]
		agaB		-		50	103							BAA04744	[49]
Vibrio sp. V134	Escherichia coli BL21	agaV	extracellular/intracellular	-		16	52	-	40	-	7.0	-	NA4, NA6	ABL06969	[8]
Agarivorans albus YKW-34	Escherichia coli DH5α	agaB34	extracellular	1670	7	16	30	242	30	50	7.0	5–9	NA4 (main)	ABW77762	[10]
Agarivorans sp. LQ48	Escherichia coli BL21	agaA	extracellular/intracellular	-		16	51	349.3	40	50	7.0	3–11	NA4, NA6	ACM50513	[41]
Pseudoalteromonas sp. CY24	Escherichia coli BL21	agaB	extracellular	17000	3	Novel	51	5000	40	35	6.0	5.7–10.6	NA8, NA10	-	[51]
α-agarase
Thalassomonas sp. JAMB-A33	Bacillus subtilis	agaA33	extracellular	6950	155	96	87	44.7	45	-	8.5	6.5–10.5	NA4 (main)	BAF44076.1	[44]