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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Bioorg Med Chem. 2009 Apr 1;17(9):3278. doi: 10.1016/j.bmc.2009.03.050

Figure 3. Effect of Triton X-100 on the inhibition of WNV protease by compound B.

Figure 3

A. The assays were performed as described under Materials and Methods in the presence and absence of Triton X-100 and 2 μM aprotinin (light grey bars) or 20 μM compound B (dark grey bars). At Triton X-100 concentrations at 0.5 CMC (CMC=0.02%), the inhibition of compound B is reduced ~85%. B. The concentrations of compound B were varied as indicated. The protease assays were performed at two different Triton X-100 concentrations (0.001 %-grey bars and 0.01%-white bars) along with no-detergent control (black bars). The experiments in panels A and B were repeated at least three times with similar results. The error bars represent standard deviation of the mean.