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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Bioorg Med Chem. 2009 Apr 1;17(9):3278. doi: 10.1016/j.bmc.2009.03.050

Table 1.

Effect of Triton X-100 on the Km and vmax values of WNV protease

Triton X-100
cone (%)		 Km
[μM]		 Vmax
[μM/min]		 kcat*
[sec−1]		 kcat/Km
10−3 [μM−1 sec−
1]
0 172 ± 60 0.97 ± 0.18 0.60 ± 0.11 3.5 ± 0.6
0.0001 182.6 ± 45 0.84 ± 0.11 0.52 ± 0.07 2.8 ± 0.4
0.001 187.1 ± 15 1.25 ± 0.25 0.77 ± 0.15 4.1 ± 0.5
0.01 240 ± 30 1.65 ± 0.20 1.02 ± 0.12 4.3 ± 0.1
0.1 220 ± 20 1.70 ± 0.01 1.05 ± 0.01 4.8 ± 0.4
*

assuming a total enzyme concentration of 27 nM

The protease assays were performed as described under Materials and Methods in the presence of indicated concentrations of Triton X-100. The Km and Vmax values were calculated using the GraphPad Prism 5.0 software.