Table 1.
A selected list of proteomic studies contributing to the understanding of neutrophil biology
| Sample/Organelle | Protein Separation | MS Analysis | Major Findings |
|---|---|---|---|
| Expression | |||
| Rat neutrophil lysate | 2DE, HPLC | MALDI-TOF, LC-ESI-MS/MS | 52 proteins identified |
| Bovine neutrophil lysate | 1DE, HPLC(RP) | LC-ESI-MS/MS | 250 proteins identified |
| Storage granules | 2DE, gel-free HPLC (RP/SCX) | 2DE/MALDI-TOF, LC-ESI-MS/MS | 2DE/MALDI identified 87 proteins ESI-MS/MS identified 247 proteins |
| Lipid rafts | 1DE | LC-FT-ICR MS/MS | 106 proteins identified |
| Lipid raft cytoskeleton | 1DE | MALDI-TOF, MS/MS | 23 proteins identified |
| Secretory vesicles and plasma membrane fractions | 1DE, free-flow electrophoresis | MALDI-TOF, HPLC-MS/MS | MALDI identified 27 proteins HPLC-MS/MS identified 80 proteins |
| Secretory vesicles and plasma membrane fractions | 2D HPLC (SCX/RP) | LC-MS/MS | 1118 total proteins identified, including 337 integral membrane proteins |
| Phagosomes | 2DE | MALDI-TOF, LC-MS/MS | 198 proteins identified, including ER machinery |
| Phagosome and plasma membrane skeletons | 2DE | MALDI-TOF | 138 proteins identified, metabolic enzymes associate with the cytoskeleton |
| Neutrophil lysate | 2D-DIGE | LC-MS/MS | FPRLR-1 ligation (de)phosphorylates cytoskeletal proteins |
| Neutrophil lysate | 2D-DIGE | MALDI-TOF/TOF | Cross-linking CD69 enhanced expression of S100A9 |
| Neutrophil lysate | 2DE | MALDI-TOF | Pharmacologic inhibition of p38 attenuated 18% of LPS-regulated proteins by ≥ 40% |
| Brochoalveolar lavage fuid | 2D-DIGE | MALDI-TOF/TOF | Identified 3 proteins having MMP2 and 9 cleavage sites: Ym1, S100A8, S100A9 |
| Structural | |||
| Neutrophil lysate | IP, 2DE | MADLI-TOF | Identified GABABR2 as an AKT substrate |
| Functional | |||
| Immunoaffinity-purified cytochrome b | 1DE | MALDI, LC-MS/MS | Reported extensive sequence coverage and PTMs for Cyt b |
| Neutrophil lysate | 1DE | MALDI | Identified substrates of MAPKAPK2, including 14-3-3ζ |
| Neutrophil lysate | 2DE | MALDI | Identified substrates of MAPKAPK2, including p16-Arc of Arp2/3 |
| Neutrophil lysate | 2DE | MALDI | Identified substrates of p38 MAPK, including MRP-14 |