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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: J Alzheimers Dis. 2008 Feb;13(1):1. doi: 10.3233/jad-2008-13101

Fig. 5.

Fig. 5

Asp664 cleavage in human entorhinal cortex. a, Representative low-magnification (100X) confocal images of hippocampal sections from patients at the indicated ages, stained with APPNeo and counterstained with TOTO-3 to visualize nuclei. Braak stage is denoted for the AD case. b and c, Total areas of APPNeo immunoreactivity in non-diseased and AD human samples were quantified as described in Methods. b, Left panel, averages ± SEM of young (20−45 Y) and aged (68−90 Y) samples are shown. Right panel, averages ± SEM of the indicated ages are shown. c, Left panel, total area of APPNeo immunoreactivity in control and AD groups. Right panel, total area of APPNeo immunoreactivity in different Braak stage groups within the AD group were compared to controls. Averages ± SEM are shown. d, Section views of a, green; b, red; c, overlay channels in stacks of confocal images collected from dorsal EC in brain sections of a 30 Y old non-diseased patient immunostained with APPNeo. f, Representative low-magnification (100X) confocal image of parahippocampal gyrus in brain sections of a 45 Y non-diseased patient immunostained with APPNeo. Sections were counterstained with TOTO-3 to visualize nucleicaption.