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LPS increases IDO enzymatic activity. Primary mouse microglia were treated with LPS (10 ng/ml) for 24 h. IDO enzymatic activity was determined by measuring the amount of KYN formed upon incubation of microglial lysates with exogenous L-tryptophan. IDO enzymatic activity was expressed as kynurenine formed per hour for per miligram of protein. Data were expressed as mean ± SEM of 3 separate experiments. ** p < 0.01 compared to control medium
