Figure 1. Phylogeny of C. gattii VGII genotypes in the United States.

This unrooted maximum likelihood tree is based on concatenated sequences from the following seven genes that are commonly used in MLST analysis of C. gattii: IGS, TEF1, GPD1, LAC1, CAP10, PLB1, and MPD1. All corresponding sequences and associated GenBank accession numbers can be found in [10,11]. The blue shaded group includes the VGIIb/minor and closely related genotypes, while the red shaded group indicates the VGIIa/major genotype. Pink coloration indicates the VGIIc novel genotype, which is closely related to the VGIIa/major genotype based on this analysis. Sequence alignments were conducted using the Clustal W software package (http://www.ebi.ac.uk/Tools/clustalw2/index.html), and the maximum likelihood tree was constructed using the PhyML software package (http://www.atgc-montpellier.fr/phyml/).