Fig. 4.
Effect of SAHA on intensity of DNA breaks and potential aberrations. A. Southern blot showing the effect of SAHA on the intensity of breaks generated at 11q23 region by anti-CD95 antibody. SAHA did not increase the number of breaks but when used in combination with anti-CD95 antibody there was a significant increase in the amount and distribution of breaks. M – Molecular weight marker. B. IPCR results of TK6 cells treated with anti-CD95 antibody with or without pre-treatment with SAHA. The possible aberrations have been shown and each band was individually cloned and sequence analyzed. M – Molecular weight marker.