Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Matrix Biol. 2009 Aug 20;29(1):22. doi: 10.1016/j.matbio.2009.08.003

Fig. 3.

Fig. 3

Effects of protease inhibitors on ECM remodeling. The experimental substratum for these studies consisted of fluorescently-labeled fibronectin layered over type I collagen. (A) PEA-10 and MEF-2 were treated with 25 µM aprotinin or with vehicle. Remodeling was allowed to occur for 3 h. (B) PEA-10 and MEF-2 cells were treated with 0.5 mM amiloride, 10 µM GM6001 or with vehicle (DMSO) and then allowed to remodel ECM for 3 h. (C) PEA-10 and MEF-2 cells were cultured on the ECM surface for 3 h in serum-free medium. Cells were permeabilized and treated with the nuclear stain, DAPI. Note that neither cell type remodeled fibronectin in the absence of serum.