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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Curr Protoc Cell Biol. 2009 Mar;CHAPTER:Unit–26.2. doi: 10.1002/0471143030.cb2602s42

Fig. 2. Localization of labeled BKV particles in HRPTEC. (A) Fluorescence of Alexa Fluor 488 labeled BKV particles in HRPTEC, (B) Staining for Endoplasmic reticulum (ER) in HRPTEC, (C) Co-localization of purified and labeled BKV with ER marker.

Fig. 2

HRPTEC were incubated with purified and labeled BKV for 6 hours. After incubation, cells were fixed and blocked. Then cells were incubated with primary antibody {1: 100 dilution of PDI (Abcam, Cambridge, MA) as ER marker against TTBS with 1 % FBS} and second antibody {1:200 dilutied Alexa flour™ 680 goat anti-mouse IgG (H+L) (Molecular Probes, Eugene, OR) in TTBS with 1 % FBS}. Cells were analyzed by confocal microscope (Leica TCS SP5) with 63× objective lens and images were captured by Leica application suite advanced fluorescence. Line is 10 µm.