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Serum OVA-specific immunoglobulin. OVA-specific (A) IgE, (B) IgG1 and (C) IgG2a determined by ELISA from sera collected 72 hours after final OVA-challenge. OVA-specific IgG1, IgG2a were undetectable for naïve mice, labeled as N.D (non-detectable). Data expressed in mean ± SEM for using 5-9 mice per group. *, p<0.05, **, p<0.01 compared to 6-week OVA-mice. w=week, M=month
