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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: Circ Res. 2009 Aug 27;105(7):639. doi: 10.1161/CIRCRESAHA.109.205120

Figure 1. S100A4/Mts1 and BMP-2 Mediated Migration of hPASMCs is Blocked by anti-RAGE and BMPRIIsi.

Figure 1

(A) Migration of hPASMCs using a modified Boyden chamber 6h without (Con) or after stimulation with S100A4 (500ng/ml) or BMP-2 (10ng/ml) ± anti-RAGE blocking antibody (1:1000; preincubation 30min). (B) Quantitative RT-PCR for BMPRII following control(con) RNAi or BMPRII RNAi. (C) Migration of hPASMCs in response to S100A4 and BMP-2 after reducing BMPRII levels by BMPRII RNAi to 20% compared to Con RNAi. (D) Migration of hPASMCs in response to PDGF-BB(PDGF) 20ng/ml using Con RNAi or BMPRII RNAi. Bars represent mean±SEM, n=6 in each group; *p<0.001, vs. control (A) or con RNAi (C, D).