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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: Circ Res. 2009 Aug 27;105(7):639. doi: 10.1161/CIRCRESAHA.109.205120

Figure 2. Phosphorylation of ERK, induced by S100A4/Mts1 and BMP-2, is necessary for migration of PASMCs downstream of RAGE, but is not BMPRII, dependent.

Figure 2

Figure 2

(A) Western immunoblot showing pERK1/2 (kDa 42/44) relative to total ERK1/2 in response to S100A4 and BMP-2. (B) Migration assays ± 30-minute preincubation with MAPK inhibitor PD 98059 (25 μM,100μM) and MEK inhibitor U0126 (10μM). DMSO as vehicle control for PD 98059. (C, D) Western immunoblot and densitometry of pERK1/2 over total ERK1/2 ± anti-RAGE ± BMPRII RNAi, un-stimulated (Con) or following stimulation with BMP-2 and S100A4 for 10 minutes. Each value is normalized to total ERK on the same blot but lanes are non-consecutive, indicated by the lines between them (C). Bars represent mean±SEM, for n=6 in B, and n=3 in C. *p<0.001 vs. Con. Experiments in C and D reflect differences in the magnitude of change in cells from different harvests.