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. Author manuscript; available in PMC: 2010 Feb 9.

Published in final edited form as: Circ Res. 2009 Aug 27;105(7):639. doi: 10.1161/CIRCRESAHA.109.205120

Western immunoblotting and densitometry of (A) active Rac1/total Rac1, and (B) active RhoA/total RhoA, from hPASMC lysates 10min to 6h after stimulation with S100A4/Mts1. (C) Immunofluorescence of MHCIIA and Rac1 in hPASMC stimulated with S100A4/Mts1 for 10 min using a primary antibody against MHCIIA and Rac1 and a secondary antibody linked to fluorescein (FITC) and rhodamine (red) under untransfected control conditions, and following transfection with non-targeting siRNA, BMPRII siRNA, and CLIC4 RNAi. Reducing CLIC4 either by BMPRII siRNA or CLIC4 siRNA reduces peripheral distribution of Rac1. Bars represent mean±SEM from n=3 experiments, *p<0.01, **p<0.001 vs. unstimulated control.

Western immunoblotting and densitometry of (A) active Rac1/total Rac1, and (B) active RhoA/total RhoA, from hPASMC lysates 10min to 6h after stimulation with S100A4/Mts1. (C) Immunofluorescence of MHCIIA and Rac1 in hPASMC stimulated with S100A4/Mts1 for 10 min using a primary antibody against MHCIIA and Rac1 and a secondary antibody linked to fluorescein (FITC) and rhodamine (red) under untransfected control conditions, and following transfection with non-targeting siRNA, BMPRII siRNA, and CLIC4 RNAi. Reducing CLIC4 either by BMPRII siRNA or CLIC4 siRNA reduces peripheral distribution of Rac1. Bars represent mean±SEM from n=3 experiments, *p<0.01, **p<0.001 vs. unstimulated control.