Experimental protocol (top) and Western blotting of SERCA isoforms (bottom). Hearts overexpressing SERCA1 and PBS controls were isolated and retrograde perfused. After an initial equilibration period, hearts underwent a 15 min no-flow ischemic insult, followed by a 50 min reperfusion period. 13C NMR spectra were acquired from hearts oxidizing 0.4 mM [2,4,6,8,10,12,14,16-13C8] palmitate and 5 mM glucose throughout reperfusion. Hearts were freeze-clamp for Western blot analysis of SERCA1, SERCA2a, and calsequestrin expression. The adenoviral group (Adv.cmv.SERCA1) demonstrated significant increases in SERCA1 expression relative to the non-viral groups.