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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 Sep 8;47(5):614. doi: 10.1016/j.yjmcc.2009.08.031

Figure 1.

Figure 1

Experimental protocol (top) and Western blotting of SERCA isoforms (bottom). Hearts overexpressing SERCA1 and PBS controls were isolated and retrograde perfused. After an initial equilibration period, hearts underwent a 15 min no-flow ischemic insult, followed by a 50 min reperfusion period. 13C NMR spectra were acquired from hearts oxidizing 0.4 mM [2,4,6,8,10,12,14,16-13C8] palmitate and 5 mM glucose throughout reperfusion. Hearts were freeze-clamp for Western blot analysis of SERCA1, SERCA2a, and calsequestrin expression. The adenoviral group (Adv.cmv.SERCA1) demonstrated significant increases in SERCA1 expression relative to the non-viral groups.