Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Bone. 2009 Sep 8;46(2):464. doi: 10.1016/j.bone.2009.08.058

Figure 3.

Figure 3

Effect of TSP2 siRNA on mineralization. A). Cells were transfected with TSP2 siRNA or a scrambled siRNA and cultured under osteogenic conditions for 21 days. Mineral nodules were stained with Alizarin Red S dye. B). The dye was solubilized using SDS and acid, and optical densities were recorded at 415 nm. Data are mean ± SEM of five independent experiments totaling 12 observations under each treatment condition. The effect of TSP2 RNAi on proliferation was monitored by direct cell counts (C) and by UV spectrophotometry (D). # p<0.001 compared to scrambled siRNA treated samples.