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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: J Cell Physiol. 2008 Oct;217(1):40. doi: 10.1002/jcp.21472

Figure 3.

Figure 3

Alkaline phosphatase activity and mineralization assays in calvarial osteoblast cells isolated from Runx2 deficient mice and wild type mice. Calvarial cells were isolated from Runx2+/+, Runx2+/− and Runx2−/− mice and were grown in osteoblastic differentiation media for up to 14 days. Compared with wild-type calvarial cells, Runx2 +/− culture showed less abundant mineralized nodules and had significantly lower Alizarin Red-S accumulation at day 14 of culture while Runx2−/− cultures showed no mineralization (A, B). Furthermore, the Runx2+/− calvarial cultures displayed significantly lower ALP activity during 14 days of culture compared with wild type mice and Runx2−/− cultures only showed background levels of ALP activity (C).