Skip to main content
. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: J Cell Physiol. 2008 Oct;217(1):40. doi: 10.1002/jcp.21472

Figure 4.

Figure 4

Heterozygous Runx2 gene knockout resulted in decreased expressions of bone marker genes and downstream transcription factor. Calvarial cells were isolated from Runx2+/+, Runx2+/− and Runx2−/− mice and were grown in osteogenic media for up to 14 days. Semiquantitative RT-PCR analyses were carried out for early and late differentiation markers as described in methods. Levels of AP, BSP, OC, OPN, Cbfa1, and OSX were normalized with those of the loading control GAPDH in three independent experiments. *p < 0.05, ** P <0.01 vs. Runx2 +/+ control cells.