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. Author manuscript; available in PMC: 2010 Jul 24.

Published in final edited form as: Adv Funct Mater. 2009 Jul 24;19(14):2325–2331. doi: 10.1002/adfm.200900107

(A) Normalized FRET as functions of acceptor (AF555-SA) concentration at constant donor (Biotin-GK(FITC)GGC) concentration (2000 nm) in PEGDA solutions or hydrogels (Asterisks designate statistical significant as per Students’ T-test performed between indicated groups; N=4, mean ± SEM; p<0.05). Noted that the normalized FRET was calibrated results based on equations provided in the Supporting Information. (B) Normalized FRET as functions of photopolymerization time at constant donor concentration (2000 nm). Straight lines were the results of linear regression and all exhibit negative slopes, indicating decline in the normalized FRET as a function of photopolymerization time. (N=4, mean ± SEM) See Table 2 for statistical analysis of the linear regression.

(A) Normalized FRET as functions of acceptor (AF555-SA) concentration at constant donor (Biotin-GK(FITC)GGC) concentration (2000 nm) in PEGDA solutions or hydrogels (Asterisks designate statistical significant as per Students’ T-test performed between indicated groups; N=4, mean ± SEM; p<0.05). Noted that the normalized FRET was calibrated results based on equations provided in the Supporting Information. (B) Normalized FRET as functions of photopolymerization time at constant donor concentration (2000 nm). Straight lines were the results of linear regression and all exhibit negative slopes, indicating decline in the normalized FRET as a function of photopolymerization time. (N=4, mean ± SEM) See Table 2 for statistical analysis of the linear regression.