Figure 2.

Alignment of amino acid sequences between previously characterized RPL10 proteins and the ORFs conserved in plant mitochondria. Upper: RPL10 from Reclinomonas mitochondrion and four eubacteria. Lower: proteins predicted from the Marchantia orf168 and its homologues in plant mitochondria. Identical or similar amino acid residues conserved in >50% sequences are highlighted. Amino acids conserved in two or more non-plant species are marked with asterisks. Gaps are indicated by dashes. Amino acid changes caused by RNA editing events, which were detected by RT–PCR analysis (Cycas, Carica, Nicotiana, Solanum, and Vitis) (this study), or computationally predicted (Megaceros) (accession no. EU660574), are denoted with red letters. Especially, amino acid positions in which the homologies were clearly improved by RNA editing are indicated with filled triangles. Positions of frame-shifts in Bambusa and Oryza are shown with slashes. Note that Brassica lacks two-thirds of the C-terminal conserved region, which is replaced by an unrelated 23 amino acid sequence (shown by lower case letters). The length of each ORF is given in parentheses.