Figure 1.
Properties of naked DNA loops attached to the NM. (A) Drawing illustrating the local topology along a typical supercoiled DNA loop that correlates with both distance relative to the NM and sensitivity to DNase I. (B) Phase contrast micrograph showing the NM of a hepatocyte nucleoid. (C) Fluorescence micrograph showing the DNA halo around the NM of a hepatocyte nucleoid caused by the unwinding of the supercoiled DNA loops by treatment of the nucleoid with the DNA-intercalating agent ethidium bromide (80 µg/ml). Scale bars 10 µm.