Fig. 3.

Phenotypes of vinculin knockdown cells are not due to altered cell-matrix adhesions. Cells were plated on coverslips coated with saturating concentrations of (A) fibronectin, (B) collagen, or (C) sub-saturating concentrations of fibronectin. The coverslips were washed, and the number of adherent cells per field of view (FOV) was counted. Thirty-five FOVs from three independent experiments were quantified and used to calculate the percent of adherent cells. Each bar represents the mean ± s.e.m.