Fig. 2.

Dicer-deficient NSCs express stem-cell markers. (A) Primary neurospheres (p-0) derived from Dicer-deficient NSCs (Ko) expressed nestin similarly to control NSCs (Ctrl). (B) More than 98% of Dicer-deficient NSCs (p-0) were BrdU positive, suggesting that they are dividing at the same rate as controls. (C) Nestin was expressed in the passaged neurospheres (p-2) derived from NSCs of KO and control cortices. The insets show neurosphere immunostaining without the primary antibodies for nestin. (D) More than 98% of Dicer-deficient NSCs (p-2) were BrdU positive. (E,F) Dissociated Dicer- deficient NSCs (p-2) expressed NSC markers such as nestin and SSEA-1. (G) Dicer-deficient NSCs (p-1) survived normally as control NSCs in a medium with mitogens. n>3 fields.