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. 2010 Jan 26;123(4):619–626. doi: 10.1242/jcs.061481

Fig. 3.

Fig. 3.

MARCH5 RING mutant induces mitochondrial elongation and cellular senescence. (A) HeLa cells were transfected with YFP vector, MARCH5-YFP and MARCH5 H43W-YFP. After incubation for 48 hours, cells were stained with MitoTracker Red and mitochondrial morphology analyzed by confocal microscopy. Boxed areas are magnified on the right. Scale bars: 10 μm. (B) Chang cells were transfected with increasing concentrations of MARCH5-YFP or MARCH5H43W-YFP expression vectors. At 48 hours post-transfection, cells were fixed and stained for SA-β-Gal activity. SA-β-Gal-positive cells were quantified. The results are the average of three independent experiments. (C) The expression levels of YFP vector, MARCH5-YFP and MARCH5H43W-YFP were analyzed by immunoblotting. *P<0.05, **P<0.01, ***P<0.001 vs control shRNA by Student's t-test.