Fig. 6.

Hepatic homogenate, microsome, mitochondria and peroxisome fraction 13′-OH-α-Tocopherol levels in response to SQ vehicle, α-tocopherol and trolox injections. (A) Total 13′-OH-α-tocopherol and (B) Percent homogenate 13′-OH-α-tocopherol were determined from SQ vehicle-, α-tocopherol and trolox-injected rats, as described in the methods. Total 13′-OH-α-tocopherol is expressed as total nmol using concentration of 13′-OH-α-tocopherol (nmol/g), starting amount of liver (g), total volume of homogenate (ml), volume of homogenate used for fractionation (ml) and final volume used to suspend subfraction pellets. All values are expressed as mean ± SE, n = 6, with * = p < 0.01 as compared with vehicle-injected rats (see methods)