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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2009 Oct 9;48(1):73. doi: 10.1016/j.freeradbiomed.2009.10.024

Table 1. Presence of organelle marker proteins in subcellular fractions isolated from vehicle-, α-tocopherol- and trolox-injected rats1.

Fraction % CYP2B1/2 % VDAC % PMP70
Microsomes 88 42 0
Mitochondria 7 96 12
Peroxisomes 5 0 88

Rats received 3 daily SQ injections of vehicle, α-tocopherol (10 mg α-tocopherol/100 g body weight) or trolox (10 mg/100 g body weight), subcellular fractions were isolated from liver homogenates and microsome (CYP2B1/2), mitochondria (VDAC), and peroxisome (PMP70) marker proteins were quantified by western analysis as described in methods.

1

Marker protein expression in subcellular fractions is expressed as percent of total expression for each protein.

2

VDAC protein was present in the microsome fraction isolated from one α-tocopherol-injected rat.