Table 1. Presence of organelle marker proteins in subcellular fractions isolated from vehicle-, α-tocopherol- and trolox-injected rats1.
Fraction | % CYP2B1/2 | % VDAC | % PMP70 |
---|---|---|---|
Microsomes | 88 | 42 | 0 |
Mitochondria | 7 | 96 | 12 |
Peroxisomes | 5 | 0 | 88 |
Rats received 3 daily SQ injections of vehicle, α-tocopherol (10 mg α-tocopherol/100 g body weight) or trolox (10 mg/100 g body weight), subcellular fractions were isolated from liver homogenates and microsome (CYP2B1/2), mitochondria (VDAC), and peroxisome (PMP70) marker proteins were quantified by western analysis as described in methods.
Marker protein expression in subcellular fractions is expressed as percent of total expression for each protein.
VDAC protein was present in the microsome fraction isolated from one α-tocopherol-injected rat.