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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Oct 27;118(1-2):41. doi: 10.1016/j.jsbmb.2009.09.016

Figure 2. Luteolin and ZN-2 Competition for Type II Sites and PC-3 Cell Inhibition.

Figure 2

Panel A. PC-3 cells were incubated (4°C × 60 min) with 40 nM [3H]estradiol plus or minus the indicated concentrations (0.004 to 40 μM) ZN-2 (black circles) or luteolin (open triangles). Specifically bound [3H]estradiol (~30,000 cpm) was determined by subtracting nonspecific cpm from the total amount of [3H]estradiol bound in the absence of competitor. Panel B. PC-3 cells were plated and allowed to attach for 48 hours. At this time (Day 0), the media was changed and the cells treated with the indicated concentrations of luteolin or ZN-2. Cell number was determined by trypan blue exclusion on Day 6.