Fig. 4.

hVPS41 overexpression does not prevent mitochondrial membrane depolarization in SH-SY5Y cells after rotenone treatment. SH-SY5Y stable cell lines were incubated under control conditions or in the presence of rotenone at indicated concentration for 48 hrs, and subsequently incubated with the mitochondrial membrane potential-sensitive dye JC-1, then quantitated with a fluorescence plate reader. There is no significant difference in mitochondrial membrane potential between vector cells and hVPS41 overexpressing lines, with or without rotenone treatment. Mean ± SD, n = 3 experiments.