Fig. 5.

hVPS41 overexpression suppresses the activation of apoptosis induced by rotenone treatment in SH-SY5Y cells. Cells were incubated in the absence or in the presence of rotenone at the indicated concentrations for 48 h prior to cell collecting (Fig. 5A, 5B). Immunoblots show that rotenone resulted in less pronounced caspase-9 and caspase-3 activation in hVPS41 overexpressing than in vector control cell lines. Measurement of caspase-3 activity assay revealed that overexpression of hVPS41-1 in SH-SY5Y cells attenuated caspase-3 activation induced by rotenone treatment. Data are expressed as fold increase over the activity in vector control cells under control conditions. Mean ± SEM. n = 3. *p < 0.05. (Fig. 5C) Cells were incubated in the absence or in the presence of 6-OHDA at the indicated concentrations for 6 h prior to cell collecting, and immunoblots showed that rotenone resulted in less pronounced caspase-9 and caspase-3 activation in hVPS41 overexpressing than in vector control cell lines. (Fig. 5D) Cells were incubated in the absence or in the presence of staurosporine at the indicated concentrations for 6 h prior to cell collecting, and immunoblots showing that staurosporine resulted in less pronounced caspase-9 and caspase-3 activation, as well as PARP cleavage in hVPS41 overexpressing than in vector control cell lines. Immunoblots shown are representative of three independent experiments.