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. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: Cell. 2009 Nov 13;139(4):757–769. doi: 10.1016/j.cell.2009.09.035

Figure 3. Flavopiridol inhibits Smad ALP.

Figure 3

(A) Cell extracts of BMP treated HaCaT cells with or without kinase inhibitors were analyzed with antibodies against the indicated proteins. SB, SB203580; SP, SP60125; combo, combination of the three MAPK inhibitors; Flavopir., Flavopiridol. (B, C) Nuclear and cytoplasmic fractions of HaCaT cells stimulated with BMP or TGFβ in the absence or presence of Flavopiridol were analyzed by immunoblotting. (D) HaCaT cells were stimulated with BMP for 1 h in the absence or presence of Flavopiridol (FP), or of Flavopiridol plus MG132. Incubations were continued for the indicated periods with media without BMP.